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Mouse Anti-Human IgG2 (Gamma 2 Chain Specific) Secondary Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS674034

MBS674034 is a secondary antibody targeting Mouse Anti-Human IgG2 (gamma 2 chain specific). Also known as Mouse Anti-Human IgG2 (Fc)-BIOT. Reactive in Human. Host: MouseSource: Ascites Fluid. More details

$325 / 0.5 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Mouse Anti-Human IgG2 (gamma 2 chain specific), Secondary Antibody
Full Product Name
Mouse Anti-Human IgG2 (Fc)-BIOT
Product Synonym Names
Mouse Anti-Human IgG2 (Fc) - Biotin; Mouse Anti-Human IgG2 (gamma 2 chain specific)
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
31-7-4
Host
Host: Mouse
Source: Ascites Fluid
Species Reactivity
Human
Form/Format
Biotin (BIOT) Conjugate
Specificity
Reacts with the Fc portion of the heavy chain of human IgG2 as demonstrated by ELISA or FLISA
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2-8 degree C or long-term at -20 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Each reagent is stable for the period shown on the bottle label if stored as directed.
Characterization
To insure lot-to-lot consistency, each batch of product is tested by ELISA for conformance to characteristics of a standard reference reagent.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
ELISA; FLISA; Immunoblot; Immunocytochemistry; Immunohistochemistry (Frozen Sections); Western Blot; Dot-and slot-imunoblotting
WORKING DILUTIONS:

FLISA:
PE conjugate 1ug/ml

ELISA:
AP conjugate 1:500-1:8,000
HRP conjugate 1:2,000-1:8,000
BIOT conjugate: 1:5,000-1:20,000


Other Applications: Since applications vary, you should dtermine the optimum working dilution of the product that is appropriate for your specific need.

Other Notes

Small volumes of Mouse Anti-Human IgG2 (gamma 2 chain specific) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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